goat anti arp 2 Search Results


anti arp2 goat polyclonal antibody  (Becton Dickinson)
90
Becton Dickinson anti arp2 goat polyclonal antibody
Colocalization of actin‐related protein 2 <t>(Arp2)</t> and WAVE2 in the cancer cells of colon carcinoma in the margin of invasion and in the metastatic site of the liver. (a) Arp2 was detected in cancer cells where budding formation was seen in the margin of invasion of the colon cancer. Bar, 250 m. (b) Digital mirror image was reconditioned to the same image of (a) by the use of computer software. WAVE2 was expressed in the same cancer cells in which Arp2 was detected in A. Bar, 250 m. (c) Arp2 was detected in the metastatic colon cancer cells in the liver of the case that showed no colocalization at the primary site. Bar, 500 m. (d) Digital mirror image was reconditioned to the same image of (c). WAVE2 is detected in the same cancer cells seen to express Arp2 in (c). Bar, 500 m.
Anti Arp2 Goat Polyclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Colocalization of actin‐related protein 2 (Arp2) and WAVE2 in the cancer cells of colon carcinoma in the margin of invasion and in the metastatic site of the liver. (a) Arp2 was detected in cancer cells where budding formation was seen in the margin of invasion of the colon cancer. Bar, 250 m. (b) Digital mirror image was reconditioned to the same image of (a) by the use of computer software. WAVE2 was expressed in the same cancer cells in which Arp2 was detected in A. Bar, 250 m. (c) Arp2 was detected in the metastatic colon cancer cells in the liver of the case that showed no colocalization at the primary site. Bar, 500 m. (d) Digital mirror image was reconditioned to the same image of (c). WAVE2 is detected in the same cancer cells seen to express Arp2 in (c). Bar, 500 m.

Journal: Cancer Science

Article Title: Correlation between liver metastasis of the colocalization of actin‐related protein 2 and 3 complex and WAVE2 in colorectal carcinoma

doi: 10.1111/j.1349-7006.2007.00488.x

Figure Lengend Snippet: Colocalization of actin‐related protein 2 (Arp2) and WAVE2 in the cancer cells of colon carcinoma in the margin of invasion and in the metastatic site of the liver. (a) Arp2 was detected in cancer cells where budding formation was seen in the margin of invasion of the colon cancer. Bar, 250 m. (b) Digital mirror image was reconditioned to the same image of (a) by the use of computer software. WAVE2 was expressed in the same cancer cells in which Arp2 was detected in A. Bar, 250 m. (c) Arp2 was detected in the metastatic colon cancer cells in the liver of the case that showed no colocalization at the primary site. Bar, 500 m. (d) Digital mirror image was reconditioned to the same image of (c). WAVE2 is detected in the same cancer cells seen to express Arp2 in (c). Bar, 500 m.

Article Snippet: First, a mixture of the anti‐Arp2 goat polyclonal antibody used for immunohistochemistry diluted to 1:50 and an anti‐Arp3 mouse monoclonal antibody (clone 4, Becton Dickinson, Franklin Lakes, NJ, USA) at a dilution of 1:1000 were prepared for double staining of Arp2 and Arp3 in order to confirm the complex formation of Ar2 and Arp3.

Techniques: Software

Immunofluorescence, Western blot analysis of actin‐related protein 2 (Arp2) and WAVE2 in colon cancer cell lines SW480 and SW620. Double staining of Arp3 and Arp2 was carried out in SW620 cells. (a) Immunofluorescence staining of Arp3 is shown as green line at the periphery of SW620 cells indicated by arrow. (b) Immunofluorescence staining of Arp2 is shown as red line at the periphery of the cells shown in (a). (c) Merged image of (a) and (b) shows colocalization of Arp3 and Arp2 as a yellow line. Triple staining of Arp3, WAVE2, and phalloidin was carried outin SW620 cells. (d) Immunofluorescence staining of Arp3 is shown as a green line at the periphery of SW620 cells indicated by arrow. (e) Immunofluorescence staining of WAVE2 is shown as a purple line at the periphery of the cells shown in (d). (f) Newly polymerized actin fibers which form lamellipodium were detected by phalloidin (blue staining). (g) Merged image of (d, e, and f) shows colocalization of Arp3, WAVE2 and phalloidin as blue‐purple staining. Bar, 20 m. (h) Expression of Arp2 and WAVE2 on the protein level by the colon cancer cell line derived from the primary site (SW480) and the corresponding cell line derived from the metastatic lymph node (SW620) were compared. WAVE2 expression was detected in both SW480 cells and SW620 cells, but the WAVE2 band detected in the SW620 cells was more distinct than in the SW480 cells. Similar results were obtained for the Arp2 expression.

Journal: Cancer Science

Article Title: Correlation between liver metastasis of the colocalization of actin‐related protein 2 and 3 complex and WAVE2 in colorectal carcinoma

doi: 10.1111/j.1349-7006.2007.00488.x

Figure Lengend Snippet: Immunofluorescence, Western blot analysis of actin‐related protein 2 (Arp2) and WAVE2 in colon cancer cell lines SW480 and SW620. Double staining of Arp3 and Arp2 was carried out in SW620 cells. (a) Immunofluorescence staining of Arp3 is shown as green line at the periphery of SW620 cells indicated by arrow. (b) Immunofluorescence staining of Arp2 is shown as red line at the periphery of the cells shown in (a). (c) Merged image of (a) and (b) shows colocalization of Arp3 and Arp2 as a yellow line. Triple staining of Arp3, WAVE2, and phalloidin was carried outin SW620 cells. (d) Immunofluorescence staining of Arp3 is shown as a green line at the periphery of SW620 cells indicated by arrow. (e) Immunofluorescence staining of WAVE2 is shown as a purple line at the periphery of the cells shown in (d). (f) Newly polymerized actin fibers which form lamellipodium were detected by phalloidin (blue staining). (g) Merged image of (d, e, and f) shows colocalization of Arp3, WAVE2 and phalloidin as blue‐purple staining. Bar, 20 m. (h) Expression of Arp2 and WAVE2 on the protein level by the colon cancer cell line derived from the primary site (SW480) and the corresponding cell line derived from the metastatic lymph node (SW620) were compared. WAVE2 expression was detected in both SW480 cells and SW620 cells, but the WAVE2 band detected in the SW620 cells was more distinct than in the SW480 cells. Similar results were obtained for the Arp2 expression.

Article Snippet: First, a mixture of the anti‐Arp2 goat polyclonal antibody used for immunohistochemistry diluted to 1:50 and an anti‐Arp3 mouse monoclonal antibody (clone 4, Becton Dickinson, Franklin Lakes, NJ, USA) at a dilution of 1:1000 were prepared for double staining of Arp2 and Arp3 in order to confirm the complex formation of Ar2 and Arp3.

Techniques: Immunofluorescence, Western Blot, Double Staining, Staining, Expressing, Derivative Assay

mRNA level expression of actin‐related protein 2 (Arp2), Arp3, and WAVE2. (a) The percentile of mRNA level of Arp2 is shown. (b) The percentile of mRNA level of Arp3 is shown. (c) The percentile of mRNA level of WAVE2 is shown. These plots patterns of percentile are similar. The Arp2 mRNA level significantly correlated with the Arp3 mRNA level (P < 0.0001). The WAVE2 mRNA level also correlated with both Arp2 (P < 0.0001) and Arp3 (P < 0.0001). (d) The average expression of normalized Arp2 at the mRNA level was 1.7 in the 35 colon cancer tissue specimens in which no Arp2 and WAVE2 colocalization was detected immunohistochemically and 5.6 in the 13 colocalization positive‐cancers. There was a significant correlation between high level of Arp2 expression and colocalization (P = 0.027). (e) In situ hybridization of Arp2 in the colon cancer tissue. Colon cancer cells expressed Arp2 stronger than adjacent non‐cancerous epithelial cells.

Journal: Cancer Science

Article Title: Correlation between liver metastasis of the colocalization of actin‐related protein 2 and 3 complex and WAVE2 in colorectal carcinoma

doi: 10.1111/j.1349-7006.2007.00488.x

Figure Lengend Snippet: mRNA level expression of actin‐related protein 2 (Arp2), Arp3, and WAVE2. (a) The percentile of mRNA level of Arp2 is shown. (b) The percentile of mRNA level of Arp3 is shown. (c) The percentile of mRNA level of WAVE2 is shown. These plots patterns of percentile are similar. The Arp2 mRNA level significantly correlated with the Arp3 mRNA level (P < 0.0001). The WAVE2 mRNA level also correlated with both Arp2 (P < 0.0001) and Arp3 (P < 0.0001). (d) The average expression of normalized Arp2 at the mRNA level was 1.7 in the 35 colon cancer tissue specimens in which no Arp2 and WAVE2 colocalization was detected immunohistochemically and 5.6 in the 13 colocalization positive‐cancers. There was a significant correlation between high level of Arp2 expression and colocalization (P = 0.027). (e) In situ hybridization of Arp2 in the colon cancer tissue. Colon cancer cells expressed Arp2 stronger than adjacent non‐cancerous epithelial cells.

Article Snippet: First, a mixture of the anti‐Arp2 goat polyclonal antibody used for immunohistochemistry diluted to 1:50 and an anti‐Arp3 mouse monoclonal antibody (clone 4, Becton Dickinson, Franklin Lakes, NJ, USA) at a dilution of 1:1000 were prepared for double staining of Arp2 and Arp3 in order to confirm the complex formation of Ar2 and Arp3.

Techniques: Expressing, In Situ Hybridization